منابع مشابه
Deamidation Kinetics of Casein Precipitated by Carbon Dioxide Compared with Commercial Caseinates
The authors are with the U.S. Dept. of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038. Direct inquiries to Dr. C.V. Santos. ABSTRACT Casein precipitated by carbon dioxide (CO2-casein), and commercial sodium and calcium caseinates (1% w/v in water) were thermally deamidated at pH 8 for up to 96h at 85, 100, and 1158C. CO2-cas...
متن کاملTransamination and associated deamidation of asparagine and glutamine.
With this system, no deamidation of glutamine occurred in the absence of a-keto acid, and, with all keto acids tested except two (pyruvate and a-ketobutyrate), transamination was absent or extremely slow when glutamate was substituted for glutamine. Experiments with W-labeled glutamine indicated that the ammonia evolved during this reaction was derived from t.he amide group of glutamine. Althou...
متن کاملEffects of glutamine deamination on glutamine deamidation in rat kidney slices.
Glutamate is known to inhibit the activity of isolated glutaminase I; however, its actual physiologic importance in regulating renal ammoniagenesis has not been established. To determine the regulatory role of glutamate on the metabolism of glutamine by rat kidney slices, we followed the effects on glutamine (2 mM) deamidation of increased removal of glutamate via augmented deamination. Three a...
متن کاملDeamidation reactions of protonated asparagine and glutamine investigated by ion spectroscopy.
RATIONALE Deamidation of Asn and Gln residues is a primary route for spontaneous post-translational protein modification. Several structures have been proposed for the deamidation products of the protonated amino acids. Here we verify these structures by ion spectroscopy, as well as the structures of parallel and sequential fragmentation products. METHODS Infrared ion spectroscopy using the f...
متن کاملGlutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family.
A family of bacterial effectors including Cif homolog from Burkholderia pseudomallei (CHBP) and Cif from Enteropathogenic Escherichia coli (EPEC) adopt a functionally important papain-like hydrolytic fold. We show here that CHBP was a potent inhibitor of the eukaryotic ubiquitination pathway. CHBP acted as a deamidase that specifically and efficiently deamidated Gln40 in ubiquitin and ubiquitin...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1986
ISSN: 0002-1369
DOI: 10.1080/00021369.1986.10867890